The World’s Brightest Minds Always Innovate At St. Jude Children’s Research Hospital, we know what can be achieved when the brightest scientific minds face the fewest barriers. That’s why we provide world-class facilities, state-of-the-art technologies, and extraordinary support for your research to ensure scientific progress is never halted from a lack of resources. At St. Jude, you will be part of a collaborative, bench-to-bedside environment where you can see, firsthand, how your science translates into survival for the children we serve. This is an exciting opportunity for a researcher to join our Center for Advanced Genome Engineering (CAGE). The CAGE has three missions: 1) facilitate investigator-initiated genome editing projects using CRISPR-Cas9, 2) perform independent R&D to evaluate and develop new genome editing techniques, and 3) to assist the gene and cell therapy programs at St. Jude Children’s Research Hospital. The Shondra Pruett-Miller laboratory offers a strong team-based environment where you will work collaboratively with other investigators across the institution to support their research needs and develop and improve genome engineering technologies. This includes creating genetically modified cell lines containing patient-specific genetic variants which enable investigators to model “diseases-in-a-dish”, tagging proteins to elucidate function and localization, and designing and creating custom CRISPR KO libraries. The CRISPR field is on the cutting edge and new technologies are being developed daily. As a member of our team, you will stay up to date on the latest genome editing techniques and incorporate them into our daily workflow to increase efficiency and offer the latest tools to St. Jude investigators. Responsibilities: Design gRNAs to create investigator specific modifications Design and implement genome editing strategies to create custom genome modified cell lines Design, implement, and troubleshoot experiments Analyze experimental data and present results at team meetings. Collaborate with other lab members and St. Jude investigators to accomplish broader goals Preferred Experience: Understanding of and experience with molecular biology techniques including DNA and RNA extractions, PCR, plasmid cloning Ability to operate in a fast-paced, multi-disciplinary environment. Comfortable working independently and as part of a team. Ability to track and execute multiple projects at once Excellent verbal and written communication skills. A general understanding of or experience with CRISPR-Cas9 technology Experience with cell culture CRISPR screening experience is a plus Computer programming and/or automated liquid handling experience is a plus Experience with Next-generation sequencing analysis is a plus Flow cytometry experience is a plus Senior Researcher Minimum Education and/or Training: Bachelor's degree in relevant scientific area is required. Minimum Experience: Two (2) years of relevant, post-degree work experience is required. No experience is required with a Master's degree. Researcher Minimum Education and/or Training: Bachelor's degree in relevant scientific area is required Minimum Experience: no experience required Compensation In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $60,320 - $105,040 per year for the role of Researcher OR Senior Researcher-Center for Advanced Genome Engineering. Explore our exceptional benefits! Diversity, Equity and Inclusion St. Jude Children’s Research Hospital has a diverse, global patient population and workforce, built on the principles of diversity, equity and inclusion. Our founder Danny Thomas envisioned a hospital that would treat children of the world—regardless of race, religion or a family’s ability to pay. Learn more about our history and commitment. Today, we continue the mission to advance cures and means of prevention for pediatric catastrophic diseases through research and treatment. As we accelerate this progress globally, we believe our legacy of diversity, equity and inclusion is foundational to success. With the commitment of leaders at all levels of the organization, we strive to ensure the St. Jude culture, leadership approaches and talent processes are equitable and culturally responsive. View our Diversity, Equity and Inclusion Report to learn about the hospital’s roots in diversity, equity and inclusion, where we are today and our aspirations for an even better future. St. Jude is an Equal Opportunity Employer No Search Firms St. Jude Children's Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.